Makhlouf Mohamed Mahmoud Bekhit
Prof. Emeritus
Identification of Salinity Tolerant Gene in Six Wheat Genotypes (Triticum aestivum) Via SSR and Sequencing Technique
J. of Agricultural Chemistry and Biotechnology, Mansoura Univ., Vol. 14 (9):117 - 123, 2023 • 2025
Publication Information
Authors
Demais, S. M. A.1; M. S. Abd EL Sabour1; M. M. M. Bekhit1*; K. I. Gad2 and T. M. S. Salim
Keywords
Triticum aestivum, antiporter gene, salinity tolerant gene, bioinformatics analysis, SSR
Journal
J. of Agricultural Chemistry and Biotechnology, Mansoura Univ., Vol. 14 (9):117 - 123, 2023
Publisher
www.jacb.journals.ekb.eg
Volume
14
Issue
9
Pages
117-123
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Six wheat genotypes which included two lines named by Line-1 and Line-2 in addition to four cultivars
(Masr-1, Shandaweel-1, Giza-171 and Sakha-94)) were used in this study.To evaluate the salt tolerant genes in
the studied wheat genotypes, 13 SSR markers were applied. Three (10%) were monomorphic, 26 (86%) were
polymorphic, and one (3.34%) produced no results. A total of 37 alleles were detected using 30 polymorphic
markers. The polymorphic information content (PIC), which found in a range of 0.00 to 0.38 on average, was
applied to assess each SSR locus's capacity to differentiate between different wheat cultivars. The significant
genetic heterogeneity of wheat genotypes was revealed by cluster analysis. Using salinity primers, amplification
of antiporter gene, and sequencing, the six genotypes were specified. Applying the BLAST bioinformatics
programe, the sequences were compared with those from the NCBI online database. According to the results, the
base size of the salinity-tolerant gene is 200 bp, compared to 600 bp for the antiporter gene(OM200013). The
phylogenetic analysis showed that the sequence of the antiporter gene was nearly typical to four gene bank
accession numbers. The antiporter gene-based phylogeny tree grouping four clades, and phylogenetic analysis
revealed 0.01 of genetic deviation at the root of each clade. As the bootstrap value between 6 and 8 %, the support
for the clades grouping was low. Moreover, as can be shown from the phylogenetic tree, the detected salinity-
tolerant gene sequences were aligned with four different NCBI website accession codes.
(Masr-1, Shandaweel-1, Giza-171 and Sakha-94)) were used in this study.To evaluate the salt tolerant genes in
the studied wheat genotypes, 13 SSR markers were applied. Three (10%) were monomorphic, 26 (86%) were
polymorphic, and one (3.34%) produced no results. A total of 37 alleles were detected using 30 polymorphic
markers. The polymorphic information content (PIC), which found in a range of 0.00 to 0.38 on average, was
applied to assess each SSR locus's capacity to differentiate between different wheat cultivars. The significant
genetic heterogeneity of wheat genotypes was revealed by cluster analysis. Using salinity primers, amplification
of antiporter gene, and sequencing, the six genotypes were specified. Applying the BLAST bioinformatics
programe, the sequences were compared with those from the NCBI online database. According to the results, the
base size of the salinity-tolerant gene is 200 bp, compared to 600 bp for the antiporter gene(OM200013). The
phylogenetic analysis showed that the sequence of the antiporter gene was nearly typical to four gene bank
accession numbers. The antiporter gene-based phylogeny tree grouping four clades, and phylogenetic analysis
revealed 0.01 of genetic deviation at the root of each clade. As the bootstrap value between 6 and 8 %, the support
for the clades grouping was low. Moreover, as can be shown from the phylogenetic tree, the detected salinity-
tolerant gene sequences were aligned with four different NCBI website accession codes.