Marwa Mohamed Ali Ibrahim Saleh
Asst. Professor
Ameliorative potential of dietary supplements, ZnO‑K, citrus essential oil, and pumpkin seed oil, on sperm quality in Nile tilapia: Insights from CASA, DNA integrity, antioxidant enzymes, and gene expressions
Fish Physiology and Biochemistry • 2025
Publication Information
Authors
Marwa M. Ali · Kamal Fathy Elboray · Engy T. Megahed · Hany T. Abu‑Taleb · Alshimaa E. Elsayed ·
Eman Y. Mohammady · Mona S. Amer · Soliman A. Morsi · Eman M. Abbas · Mohamed S. Hassaan ·
Hosam Elsaied
Keywords
Antioxidant supplements · CASA
parameters · DNA integrity · Enzyme bioassay · Gene
expression · O. niloticus · Sperm quality
Journal
Fish Physiology and Biochemistry
Publisher
Springer Netherlands
Volume
51:114
Issue
4
Pages
1-15
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Abstract Improving sperm quality is essential for
enhancing the reproductive efciency of Oreochromis
niloticus. This study evaluated the efect of three dietary antioxidant supplements, kaolinite-doped zinc
oxide (ZnO-K), citrus essential oil (CEO), and pumpkin seed oil (PSO), on sperm quality parameters. A
suite of integrative assessments, including ComputerAssisted Semen Analysis (CASA) parameters, spermatozoa DNA integrity, antioxidant enzyme assays,
and gene expression profling, was applied to validate
sperm quality. A total of 192 adult males (mean
weight 421.31 g±30.41 SD) were assigned to four
dietary groups, each with three replicates. The control
group received a basal diet without supplementation.
The experimental groups were fed diets supplemented
with ZnO-K (0.06 g kg⁻
1
), CEO (10 g kg⁻
1
), or PSO
(15 g kg⁻
1
). ZnO-K supplementation signifcantly
(P
enhancing the reproductive efciency of Oreochromis
niloticus. This study evaluated the efect of three dietary antioxidant supplements, kaolinite-doped zinc
oxide (ZnO-K), citrus essential oil (CEO), and pumpkin seed oil (PSO), on sperm quality parameters. A
suite of integrative assessments, including ComputerAssisted Semen Analysis (CASA) parameters, spermatozoa DNA integrity, antioxidant enzyme assays,
and gene expression profling, was applied to validate
sperm quality. A total of 192 adult males (mean
weight 421.31 g±30.41 SD) were assigned to four
dietary groups, each with three replicates. The control
group received a basal diet without supplementation.
The experimental groups were fed diets supplemented
with ZnO-K (0.06 g kg⁻
1
), CEO (10 g kg⁻
1
), or PSO
(15 g kg⁻
1
). ZnO-K supplementation signifcantly
(P