mohamed ahmed abdelaziz elashry
Asst. Professor
Synergistic effects of Bacillus pumilus and exogenous protease on Nile tilapia (Oreochromis niloticus) growth, gut microbes, immune response and gene expression fed plant diet
Animal Feed Science and Technology • 2021
Publication Information
Authors
Hosam E Elsaied Mohamed S Hassaan, Eman Y Mohammady, Mohamed R Soaudy, Mohamed A Elashry, Mahmoud MA Moustafa, Mai A Wassel, Hoda AS El-Garhy, Ehab R El-Haroun
Keywords
Exogenous enzymes, Nile tilapia
Journal
Animal Feed Science and Technology
Publisher
Elsevier
Volume
275
Issue
Not Available
Pages
114892
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The synergistic effects of probiotic (Bacillus pumilus) and/or exogenous enzyme (protease) on growth, immunity, serum parameters, gene expression and gut bacteria of Nile tilapia (3.62 ± 0.06 g) were investigated. Plant protein-based diets were formulated as the control diet, probiotic (pro, 1.85 × 105 B. pumilus CFU kg−1), protease enzyme (enzy, 0.5 g protease kg −1), and their mixture of 1.85 × 105 B. pumilus CFU kg−1 + 0.5 g protease kg −1 (pro-enzy), respectively. After 84 days, the results revealed that fish fed pro-enzy diet resulted in better (P < 0.05) growth performance and feed utilization, including highest goblet cells, thickness of musclaris, mucosal folds and enterocytes. In addition, the highest values of hematocrit (Htc), hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs), immunoglobulin M (IgM), lysozyme and phagocytic and serum parameters, including albumin, globulin and total …