Mohammed Ahmed El Sayed Kassem
Professor
Determination of Nano Amounts of Copper in Environmental, Biological, and Water Samples Via Cloud Point Extraction and Spectrophotometry
Analytical Chemistry Letters • 2017
Publication Information
Authors
Alaa S. Amin & Mohammed A. Kassem
Keywords
Copper determination, Cloud point extraction, Spectrophotometry, Triton X-114,
Azo compound, Environmental, Water and biological analysis.
Journal
Analytical Chemistry Letters
Publisher
Taylor and francis
Volume
6
Issue
6
Pages
820 - 833
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A cloud point extraction procedure was presented for the preconcentration and determination
of copper(II) ion in various real samples. After complexation by amino-4-(3-nitrophenylazo)pyridine-3-ol,
(ANPAP), copper(II) ions was quantitatively recovered in Triton X-114 after centrifugation. 0.4 mL of ethanol
acidified with 1.0 M HNO3 was added to the surfactant-rich phase prior to its analysis by specrophotometry at
λmax 618. The influence of analytical parameters including complexing agent, Triton X-114, buffer solutions,
bath temperature, heating time, centrifuge rate and time were optimized. Under the optimum conditions, the
calibration curve is linear for the concentration range of 4.0-115 ng mL-1. The detection and quantification
limits of the method were 1.20 and 3.94 ng mL-1 of Cu(II) ion. The enhancement factor of 250 was achieved for
100 mL samples containing the analyte with RSDs were ³1.25 %. The interference effect of some anions and
cations was also tested. The method was applied to determine of copper in environmental, water and biological
samples.
of copper(II) ion in various real samples. After complexation by amino-4-(3-nitrophenylazo)pyridine-3-ol,
(ANPAP), copper(II) ions was quantitatively recovered in Triton X-114 after centrifugation. 0.4 mL of ethanol
acidified with 1.0 M HNO3 was added to the surfactant-rich phase prior to its analysis by specrophotometry at
λmax 618. The influence of analytical parameters including complexing agent, Triton X-114, buffer solutions,
bath temperature, heating time, centrifuge rate and time were optimized. Under the optimum conditions, the
calibration curve is linear for the concentration range of 4.0-115 ng mL-1. The detection and quantification
limits of the method were 1.20 and 3.94 ng mL-1 of Cu(II) ion. The enhancement factor of 250 was achieved for
100 mL samples containing the analyte with RSDs were ³1.25 %. The interference effect of some anions and
cations was also tested. The method was applied to determine of copper in environmental, water and biological
samples.