NEHAL FAHMY SHAHEEN
Asst. Professor
Effects of Insulin and Resveratrol versus Mesenchymal Stem Cells (MSCs) on the Pancreas of Diabetic Rats (Type-I) (Light and Electron Microscope Study)
benha medical journal • 2025
Publication Information
Authors
Samia M. Manawy , Essam M.Eid, Ali M. Ali, Fatma M. Ahmed, Nehal F.Shaheen
Keywords
AD-MSCs, type1 DM, Resveratrol, Insulin
Journal
benha medical journal
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
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publication.type
Local
Paper Link
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Supplementary Materials
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Abstract
Abstract:
Background: transplantation of mesenchymal stem cells (MSCs) provides an effective strategy to protect pancreatic tissue injury in diabetes type 1. Many factors act as obstacles to their best regenerative capacity. Aim of the work: To evaluate the role of Adipose-derived mesenchymal stem cell (AD-MSCs) in the treatment of type 1 DM and to compare their efficacy alone and with a combination with resveratrol and long-acting insulin in the pancreas of albino rats with streptozotocin induced T1DM. Methods: 10 out of the 50 healthy adult male albino rats served as donors for stem cells and the other 40 rats were subdivided into two main groups: Group I (control group (16 rats), Group II (STZ-diabetic group, (24) rats which were injected with a single intraperitoneal injection of streptozotocin 50 mg/kg BW. This group was subdivided into 4 groups. GII a: untreated rats, GII b: received 1x10⁶ of cultured rat ADMSCs once intravenously into the tail vein, GII c which was given MSCs plus resveratrol in combination, GII d which was given MSCs & resveratrol and insulin glargine. Pancreatic samples were prepared for immune histochemical study, light and electron microscopic examination, and morphometric studies. Results: The AD-MSCs-treated group revealed moderate improvement in histological structure and fasting blood glucose level. AD-MSCs, resveratrol and insulin-treated group demonstrated higher improvement than that of the MSCs treated group alone or dual combination of AD-MSCs plus resveratrol. Conclusion: Better synergistic protective effect on damaged pancreatic tissues can be observed in STZ induced DM rats treated with AD- MSC coupling with resveratrol and insulin treatment.
Background: transplantation of mesenchymal stem cells (MSCs) provides an effective strategy to protect pancreatic tissue injury in diabetes type 1. Many factors act as obstacles to their best regenerative capacity. Aim of the work: To evaluate the role of Adipose-derived mesenchymal stem cell (AD-MSCs) in the treatment of type 1 DM and to compare their efficacy alone and with a combination with resveratrol and long-acting insulin in the pancreas of albino rats with streptozotocin induced T1DM. Methods: 10 out of the 50 healthy adult male albino rats served as donors for stem cells and the other 40 rats were subdivided into two main groups: Group I (control group (16 rats), Group II (STZ-diabetic group, (24) rats which were injected with a single intraperitoneal injection of streptozotocin 50 mg/kg BW. This group was subdivided into 4 groups. GII a: untreated rats, GII b: received 1x10⁶ of cultured rat ADMSCs once intravenously into the tail vein, GII c which was given MSCs plus resveratrol in combination, GII d which was given MSCs & resveratrol and insulin glargine. Pancreatic samples were prepared for immune histochemical study, light and electron microscopic examination, and morphometric studies. Results: The AD-MSCs-treated group revealed moderate improvement in histological structure and fasting blood glucose level. AD-MSCs, resveratrol and insulin-treated group demonstrated higher improvement than that of the MSCs treated group alone or dual combination of AD-MSCs plus resveratrol. Conclusion: Better synergistic protective effect on damaged pancreatic tissues can be observed in STZ induced DM rats treated with AD- MSC coupling with resveratrol and insulin treatment.