Context: Plasmapheresis considered as safe, fast and powerful therapeutic approach for
chronic disease management through removing destructive antibodies and toxins from patient's blood.
Aim: to evaluate how educational guidelines implementation can affect nurses’ knowledge and
practice regarding plasmapheresis process. Methods: The study used a quasi-experimental design,
specifically employing a pre/post-test methodology on a convenience sample of (60) nurses who
assigned to care for patients who underwent to plasmapheresis. This study was conducted in the
hemodialysis unit at Benda University Hospital. The study utilized the following tools: I) Selfadministered questionnaire consisting of nurses’ personal data and knowledge assessment sheet. II)
Observational checklist to evaluate nurses’ skills before, during, after plasmapheresis. Results:
16.7% of studied nurses had satisfactory level of total knowledge about plasmapheresis process pre
guidelines implementation to be significantly improved during immediate and post three months of
guidelines implementation among 76.7% & 66.7% respectively. While 20.0% of studied nurses had
satisfactory level of total practice about plasmapheresis process pre guidelines implementation to be
significantly improved during immediate and post three months of guidelines implementation among
70.0% & 63.3%, respectively. There was a highly significant difference in total nurses’ knowledge
and practice score within study periods observed as p