This study aimed to investigate the wound healing effects of peppermint (P) single and its co-emulsion with black seed oil (B) or frankincense oil (F) through an ultrasonication process. Successfully Prepared nano-emulsions were examined through TEM, particle size analyser (PSA), and zeta potential (ZP) techniques. The produced emulsion was applied to non-woven fabric (NWF) by spraying technique. Non-woven fabric surface morphology was produced and examined through a scanning electron microscope (SEM). Antibacterial activities of emulsions treated NWF were examined against Staphylococcus aureus as gram-positive bacteria and Escherichia coli as gram-negative bacteria and their healing effect to second burn degree were examined through reduction of the burn size. Additionally, Histopathological evaluations also evaluated for burn skin samples. Nano-emulsion droplets size lies between 20 and 70 nm and are increased by storage. The Zeta potential of P/F emulsion showed the highest negative charge compared with all prepared emulsions. FTIR-ATR charts showed new peaks for oils treated NWF compared with untreated NWF sample. The P/F emulsion showed higher zeta potential measurement compared with other single or Co-emulsions. For burn healing in-vivo, P/F emulsion treated NWF showed complete healing with original skin colour compared with other groups. While histopathological evaluation showed a homogenous epidermis with more granulation tissue formation than P/B emulsion treated NWF and all single oils emulsions. Antimicrobial evaluation of co-emulsions showed higher results than single oils emulsions. The results of co-emulsion oils showed superior properties and burn healing, especially P/F co-emulsion.