Context: Genitourinary anomalies account for the most common urogenital disorders in children. Therefore, pediatric nursing excellence model will continue to be central to excellence in nursing practice andoptimalchildcare. Aim: To evaluate the effect pediatric nursing excellence model on nurses' knowledge andpracticeregarding care of children undergoing surgical repair of genitourinary anomalies. Research design: Researchersutilize a quasi-experimental research. Setting: The present study was conducted at the inpatient pediatricsurgical department, at Benha Specialized Pediatric Hospital in Benha city. Study sample: All availablenurses, they composed of 60 nurses working at the previously mentioned settings were included in the current studyregardless of their personal characteristics. Study tools: Three tools were used for data collection, Tool I:Astructured interviewing questionnaire developed to assess nurses’ personal characteristics andlevel ofknοwledge regarding care of children undergoing surgical repair of genitourinary anomalies. Tool II: Anobservational checklist to assess nurses' practice regarding care of children undergoing surgical repair ofgenitourinary anomalies Tool III: A structured questionnaire based on Pediatric Nursing Excellence(PNE)model regarding care of children undergoing surgical repair of genitourinary anomalies. Results: The majorityof the studied nurses had satisfactory level of knowledge and competent level regarding their total practicepostPNE model application. Also, the majority of them had high excellence level post PNE model application, whileless than three-quarters had low excellence level pre PNE model application. Conclusion: It could be concludedthat nurses’ level of knowledge and practice improved regarding the care of children undergoing surgical repairof genitourinary anomalies, which emphasize that PNE model application was effective. Recommendation:Developing strategies to improve nursing care for children undergoing surgical repair of genitourinaryanomaliesis needed to achieve high competent nurses’ performance