Background: Human papillomavirus (HPV) is the commonest viral sexually transmitted infection in the world and the leading cause of cervical cancer. Aim: of this study was to evaluate the effect of educational intervention on knowledge and attitudes regarding human papillomavirus infection and its vaccination among nursing students. Design: Quasi-experimental design was utilized. Sample: A purposive sample of 200 female nursing students who registered in the first academic year at Faculty of Nursing Benha University. Setting: The study was carried out at Faculty of Nursing Benha University. Tools: Data were collected through two main tools: A self-administered questionnaire to assess students’ general characteristics and knowledge regarding HPV infection and its vaccination, and Modified Likert’s Scale to assess students’ attitude regarding HPV infection and its vaccination. Results: showed that the mean age of studied sample 17.87±0551years. There was improvement with highly statistically significant difference observed in students’ knowledge and attitude regarding HPV infection and its vaccination at post-intervention phase compared with pre-intervention phase (p 0.05). While, there was a highly positive statistical significant correlation between total knowledge and total attitude regarding human papilloma virus infection and its vaccination at post-intervention phase (p