Samy Ali Hussein Aziza
Prof. Emeritus
Preparation and Serological Evaluation of an Inactivated Trivalent Oil Emulsion Vaccine for Avian Fowl Adenovirus (FAdV) Containing − 8a, 8b, and 11 Serotypes
Current Microbiology • 2026
Publication Information
Authors
Ferial Eliwa Ibrahim1 · Mohamed Nasr Fathi Shaheen1,2 · Hasnaa Maged1 · Mahmoud Ibrahim1,3 ·
Yakout Abdelfatah EL-Senosi4 · Samy Ali Hussein Aziza4 · Afaf Desoky Abdel Magid4 · Ahmed El-Sanousi1,
Keywords
IBH , FAdV , Vaccine , Immunization , Chicken
Journal
Current Microbiology
Publisher
Springer
Volume
83
Issue
Not Available
Pages
147
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Inclusion body hepatitis (IBH), caused by fowl adenovirus (FAdV) species D and E, results in significant economic losses
for the poultry industry. This study aimed to evaluate the immunogenicity of an inactivated trivalent FAdV vaccine in
SPF chickens, specifically assessing the effects of antigen payload, thimerosal addition, and booster immunization on antibody
titers. Results demonstrated a clear dose-response, with higher antigen payloads eliciting a more prolonged immune
response compared to the lowest dose. Thimerosal was confirmed to be a safe preservative, showing no negative impact
on antibody development. Furthermore, a booster immunization significantly increased antibody levels, but this effect was
observed only in the group receiving the high-payload vaccine, with peak titers at weeks 2 and 3 post-vaccination. These
findings indicate that a high-dose trivalent inactivated FAdV vaccine, administered with a booster, is highly immunogenic
and represents a promising strategy for the effective prevention and control of IBH.
for the poultry industry. This study aimed to evaluate the immunogenicity of an inactivated trivalent FAdV vaccine in
SPF chickens, specifically assessing the effects of antigen payload, thimerosal addition, and booster immunization on antibody
titers. Results demonstrated a clear dose-response, with higher antigen payloads eliciting a more prolonged immune
response compared to the lowest dose. Thimerosal was confirmed to be a safe preservative, showing no negative impact
on antibody development. Furthermore, a booster immunization significantly increased antibody levels, but this effect was
observed only in the group receiving the high-payload vaccine, with peak titers at weeks 2 and 3 post-vaccination. These
findings indicate that a high-dose trivalent inactivated FAdV vaccine, administered with a booster, is highly immunogenic
and represents a promising strategy for the effective prevention and control of IBH.