Adolescence is a crucial time of physical and psychological changes in which young people begin to establish social and unique identities. The research aimed to evaluate the impact of the adaptation model on adolescent students' self-concept and promote health behavior during puberty. This research was conducted as quasi-experimental design done at secondary nursing school in teaching hospital at Benha city. A convenient sample of 113 adolescent students was included in this research. Four data collection tools were utilized, including a structured interviewing questionnaire, Adolescents’ Self-concept Short Scale, Puberty health behavior questionnaire, and Roy's Adaptation Model construct scale. Results of this research revealed that there was a highly statistically significant difference in all items of studied adolescent students’ self-concept and healthy behaviors during puberty before and after four months of adaptation model application (p ≤ 0.001). This research concluded that application of Roy’s Adaptation Model was effective in improving self-concept and enhancing promoted healthy behavior among adolescent students during puberty. This research proposes to benefit from the positive impact of this model as a guide for advancing care training programs to face challenges of adaptation in this period.