الشيماء الحسيني حسب النبي حسب النبي
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Impact of nano‑selenium on nuclear maturation and genes expression profile of buffalo oocytes matured in vitro
20 Oct 2020Abstract Supplementation of maturation media with antioxidant (bulk form) improves oocyte maturation. However, the influence of adding antioxidant (nano-particles) on oocyte maturation is not well known. We aimed to evaluate the effect of selenium...
Download AttachmentInfluence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development
20 Oct 2020R IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. T...
Effect of Cumulus Cells and Meiotic Stages on Survivability and Meiotic Competence in Vitrified Buffalo Oocytes
20 Oct 2020Abstract.- Cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. Buffalo oocytes are sensitive to chilling injury, making their cryopreservation very difficult. To improve the efficacy o...
Establishment a protocol for total RNA isolation from buffalo fresh and frozen semen for molecular applications
20 Oct 2020Abstract To date, there is no an established protocol for total RNA isolation in Egyptian buffalo spermatozoa. The present study aimed (I) to establish a defined protocol for total RNA isolation from fresh and frozen spermatozoa, (II) to evaluate...
Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection
02 Jan 2018T HE SUCCESS of in vitro embryo production is ultimately dependent on the number and quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria are routinely used for oocytes selection in most laboratories. There is brilli...
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