animals and humans they are associated with nosocomial infections worldwide. We investigated the occurrence and vancomycin resistance of enterococci in poultry Methods: A total of 617 cloacal swabs were collected from diseased poultry diagnosed with gastrointestinal disorders in 6 districts in Egypt. Isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The antibiotic susceptibility testing of all isolates against ticoplanin and vancomycin antibiotics was performed with the MICRONAUT system for Grampositive bacteria. The presence of 3 resistance-associated genes vanA, vanB and vanC1 was investigated by PCR. Results: Four species were identified: Enterococcus faecium (n= 30), Enterococcus faecalis (n= 16), Enterococcus gallinarum (n= 10) and Enterococcus avaim (n= 8) strains. Antimicrobial resistance profiles of enterococci isolates could be determined (Table 2). Resistance rate to vancomycin were different. E. feacalis showed high resistantance rate to both teicoplanin 75.0% and vancomycin 87.5%, followed by E. faecium that showed resistance to teicoplanin 40.0% and vancomycin 50.0% and E. gallinarum resistance to teicoplanin was 40.0% and vancomycin 30.0% while E, avaim showed the lowest rate of resistance to teicoplanin was 25.0% and vancomycin 25.0%. Vancomycin resistance genes were found in 14 isolates. The vanA were detected in ten isolates of E. feacalis only. The vanB gene was identified in three E. faecium isolates and five isolates of E. feacalis. The …