Background: Cardiac dyshomeostasis is steadily increasing in obese diabetics, with growing
evidence pointing to a potential connection between diabetic cardiomyopathy (DCM) and gut
microbiota alterations. The probiotic Lactobacillus acidophilus (Lacto-B) has garnered significant
interest for its beneficial properties. This study aimed to explore the mechanisms by which LactoB exerts its protective effects and to assess the role of beclin-1-mediated autophagy in DCM.
Method: Twenty-four rats were grouped into Control, Lacto-B, high-fat diet (HFD), and HFD +
Lacto-B, for 8-week experimental period. Lacto-B was administrated at a dose of (2 x 108
)
colony-forming units/ml/d by oral gavage. Echocardiographic, biochemical, and microscopic
evaluations for cardiac changes were assessed. Glycemic and lipid profiles, oxidative stress,
inflammatory markers nuclear factor-кB, interleukin-1β, interleukin-10, and beclin-1 protein were
evaluated